Community-Level Physiological Profiling Performed with an Oxygen-Sensitive Fluorophore in a Microtiter Plate
نویسندگان
چکیده
منابع مشابه
Online monitoring of dissolved oxygen tension in microtiter plates based on infrared fluorescent oxygen-sensitive nanoparticles
BACKGROUND During the past years, new high-throughput screening systems with capabilities of online monitoring turned out to be powerful tools for the characterization of microbial cell cultures. These systems are often easy to use, offer economic advantages compared to larger systems and allow to determine many important process parameters within short time. Fluorescent protein tags tremendous...
متن کاملMicrotiter plate immunoenzymometric assay for estrogen receptor.
The estrogen receptor (ER) status of breast cancer is used both as a prognostic factor and as a predictor of response to endocrine therapy. An immunoenzymometric assay for ER was developed on 96-well microtiter plates (EIA96). This technique involves two monoclonal antibodies directed against different epitopes in the B domain of ER. The two-step protocol (16-18 h and 3 h at 4 degrees C) requir...
متن کاملCommunity-level physiological profiling analyses show potential to identify the copiotrophic bacteria present in soil environments
Community-level physiological profiling (CLPP) analyses from very diverse environments are frequently used with the aim of characterizing the metabolic versatility of whole environmental bacterial communities. While the limitations of the methodology for the characterization of whole communities are well known, we propose that CLPP combined with high-throughput sequencing and qPCR can be utiliz...
متن کاملMicrotiter plate agglutination test for Salmonella antibodies.
Similar results were obtained when testing human sera for Salmonella antibodies by the tube agglutination test and by the Microtiter plate agglutination test. The plate test was easier to perform and saved time, space, antigen, and serum.
متن کاملA Microtiter Plate-Based Assay for Inhibitors of Penicillin-Binding
PBP2a, the molecular determinant for high level β-lactam resistance in 19 Methicillin-resistant Staphylococcus aureus (MRSA), is intrinsically resistant to most β20 lactam antibiotics. The development and characterization of new inhibitors targeting 21 PBP2a would benefit from an effective and convenient assay for inhibitor binding. This 22 study was directed towards the development of a fluore...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.5.2994-2998.2003